ASTM E Antimicrobial Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents under Dynamic Contact Conditions. The test.
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It is for this reason that the use of the term leaching throughout this document is limited to only the testing conditions described herein. This test ensures good contact between bacteria and the treated fibre by constant agitation of the test specimen in a bacterial suspension during the test period 1hour. The test quantitatively evaluates the effectiveness of a sample treated with a non-leaching antimicrobial agent by shaking in an organism suspension. The number of viable organisms from the suspension is determined and the percent reduction is calculated by comparing retrievals from appropriate controls.
Link to Adtm This link will always route to e2194 current Active version of the standard. Referenced Documents purchase separately The documents listed below are referenced within the subject standard but aztm not provided as part of the standard.
ASTM E – Method Determining the Antimicrobial Activity
This dynamic shake flask test was developed for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials. Presence of residual antimicrobial activity indicates the presence of a leaching type of antimicrobial agent.
No other units of measurement are included in this standard. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. The antimicrobial activity of a substrate-bound, non-leaching antimicrobial agent is dependent upon direct contact of microbes with the active chemical agent.
This test method ensures good contact between aatm bacteria and the treated fiber, fabric, or other substrate, by constant agitation of the test specimen in a challenge suspension ee2149 the test period. Link to Active This link will always route to the current Active version of the standard. Organisms typically used in this method: Note 1 — Stresses may include laundry, wear and abrasion, radiation and steam sterilization, UV exposure, solvent manipulation, temperature susceptibility, or similar physical or chemical manipulation.
We use this information to improve and customize your browsing experience and for analytics and metrics about our visitors — both on this website and other media. Record asrm report presence of solution activity. This test method standardizes both the growth conditions of the challenge species and substrate contact times to reduce the variability associated with growth phase of the microorganism.
It has been developed for routine quality control and screening tests and is used to evaluate substrate bound antimicrobials. Summary of Test Method: In this method, treated test samples are placed in a laboratory flask containing a dilute asm of test organism, commonly Escherichia coli.
Historical Version s – view previous versions of standard. If an antimicrobial agent that is shown to be removed from the surface by Section 13 is utilized in this test methodology, xstm must be included such that appropriate neutralization steps are including during recovery and enumeration.
The ASTM E Standard Test R2149 is used to evaluate the antimicrobial activity of substrate-bound, non-leaching, antimicrobial-treated samples under dynamic contact conditions.
ASTM E – Microbe Investigations (MIS)
This test method is designed to evaluate the antimicrobial activity of non-leaching, antimicrobial-treated specimens under dynamic contact conditions. The presence of a leaching antimicrobial is determined post-test. This website stores cookies on your computer.
Materials evaluated by this method may be comprised of fabric, paper, powder or other solid materials.
To determine if a compound is immobilized in all conditions or during the end use of the product additional testing may be required. Work Item s d2149 proposed revisions of this standard. Work Item s – proposed revisions of this standard.
ASTM E2149 Antimicrobial Activity
Surface antimicrobial activity is determined by comparing results from the test sample to controls run simultaneously. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For example, water soluble antimicrobials will be prone to removal from the test surface using the method described in Section 13 but insoluble compounds will not.
Test Package Request Form. No regulated reduction limits currently exist for general antimicrobial claims made using this method.
Referenced Documents purchase separately The documents listed below are referenced within the subject standard but are not provided as part of the standard. This test method is only intended to determine efficacy as described in 4. All the samples are shaken in dynamic shake flasks in a wrist action shaker to ensure good contact between the bacteria and the treated fibre, fabric, or other substrate by constant agitation of the test specimen in a bacterial suspension during the test period.
The resulting plates are incubated, the number of survivors is enumerated and a percent reduction is determined for the test flask as compared to the untreated control suspension. This dynamic shake flask test was developed for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials.
Active view current version of standard.
The flask is placed onto a wrist-action shaker and shaken for a desired exposure time, typically 1 hour. This method is generally used to substantiate antimicrobial properties of treated specimens for non-public health related claims.
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Immobilized antimicrobial agents, such as surface bonded materials, are not free to diffuse into their environment under normal conditions of use. These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately applied static conditions as in AATCCsensitivity, and reproducibility.
Microbial concentrations in the treated and untreated samples are usually determined at 0, 1 or 24 hours contact time. The susceptibility of the species to particular biocides could be altered depending on its life stage cycle. This dynamic shake flash test was developed for routine quality control and screening test.
The test determines the antimicrobial activity of a treated specimen by shaking samples of surface-bound materials in a concentrated bacterial suspension for a one hour contact time. One-hour contact time in a buffer solution allows for metabolic stasis in the population.