API (Analytical Profile Index) 20E presented is a biochemical panel for identification and differentiation of members of the family. Changes to thesaurus. Changes to database old new. Taxons. Notes. Identification. Additional tests. API® 20 E v v X. X. X. X. RapiD 20 E™ v v X. API® 20 Microbial Identification Kits, bioMerieux. Supplier: Quantity. API® 20 E Microbial Identification Kit, , , , Pack of 25, Retrieving.
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The 12 substrates contained in the 12A strips are available in a solid microplate format, referred to as 12E. Using the results obtained, from each group of three reactions a 9 nine digit code number is produced.
Refer to the procedure chart for a condensed version of the following procedures. Argine dihydrolase converts arginine into ornithine, ammonia and carbon dioxide.
Organism identification is based on pH change and substrate utilisations as established by published reference methodologies 4,6,8,9.
Bromothymol blue indicates formation of the specific amine cadaverine.
Citrate utilization citrate is the only source of carbon. General Aspects and Perspectives. Prolonged incubation, insufficient ggalerie, improper filling of wells, or inadequate inoculum may lead to false results.
The interpretation of mathematically calculated identification results requires trained clinical personnel who should use judgement and knowledge in conjunction with the following information before accepting the ID of an organism: This code is entered into the computer package.
Solid gelatin particles which may drift across the well after rehydration should be considered as a negative reaction. Record all positive results.
API for Microorganism Identification | bioMérieux
Indole Kovacs reagent forms a pink-red complex with indole. Hydrolysis of gelatin is indicated by dispersal of the black particles throughout the well.
Using a sterile pipette or dropper bottle, overlay the substrates underlined on the holding tray with sterile mineral oil, i. Inoculation The wells of individual substrate sets can be exposed by cutting the end tag apk the sealing strip and slowly peeling it back.
Some bacterial strains may have atypical biochemical reactions due to unusual nutritional requirements or mutations and may be difficult to identify. Mix thoroughly to prepare a homogeneous suspension.
Identification of Pseudomonas aeruginosa with the API-20E system.
Quality control The overall performance of the system should be monitored by testing appropriate control strains. This will determine whether nitrate has been reduced completely to nitrogen gas N2. Warning and precautions 1. These can be purchased individually or as a set by ordering product code MB A Gram-stain and oxidase test should be performed prior to set-up of tests. Twelve substrates provide insufficient data to speciate within this group as a single aberrant reaction may result in an incorrect identification.
Bromothymol blue indicator changes from blue to yellow when the carbohydrate is utilised to form acid. Well 8 Indole production – add 2 drops of Indole Kovacs reagent. When Actinobacillus or Pasteurella spp. H 2 S reacts with ferric salts in the medium to form a black precipitate. The 24E solid microplate format contains the 24 substrates contained in the combination of both the 12A and 12B strips.
BIOMIC V3 Microbiology System – Automated API Reader – Giles Scientific USA
Material Safety Data Sheet. The lysine and ornithine decarboxylase reactions should be carefully interpreted. The expected results is positive.
Each group of three reactions produces a single digit of the code.
The results should be interpreted as follows:. Green reactions occurring at 48 hours should be interpreted as negative. MBA Microbact 12E 20 pouches, identifications. All systems should be read after 48 hours for the identification of Miscellaneous Gram-negative bacilli.
Production of a red colour within a few minutes of the addition of the reagent indicates that nitrate reduction to nitrite NO 2 has occurred. The arginine reaction well 12 for 12B and well 24 of 24E is interpreted differently at 24 hours and 48 hours gaelrie. Bromothymol blue is the indicator. This causes a pH rise as indicated by bromothymol blue. The 12A strip may be used alone for identification of oxidase-negative, nitrate-positive glucose fermenters comprising 15 genera and may be useful for screening pathogenic Enterobacteriaceae from enteric and urine specimens or identification of other common isolates.
For aid in interpreting reactions, refer to the Table of Reactions. Preparation of inoculum Pick isolated colonies from an galfrie culture and emulsify in 2.